LOCUS       40924_5209       12677 bp DNA     circular SYN 17-DEC-2018
DEFINITION  PiggyBac transformation vector pB-UGIR w+, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12677)
  AUTHORS   Griswold CM, Roebuck J, Andersen RO, Stam LF, Spana EP.
  TITLE     A toolkit for transformation and mutagenesis in Drosophila using 
            piggyBac
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12677)
  AUTHORS   Griswold CM, Roebuck J, Andersen RO, Stam LF, Spana EP.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2002) Invertebrate Targets, Syngenta 
            Biotechnology, Inc., 3054 Cornwallis Rd, Research Triangle Park, NC 
            27709, USA
REFERENCE   3  (bases 1 to 12677)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12677)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-DEC-2002) Invertebrate Targets, Syngenta Biotechnology, Inc., 
            3054 Cornwallis Rd, Research Triangle Park, NC 27709, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12677
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  complement(11..620)
                     /mobile_element_type="transposon:piggyBac 
                     transposableelement"
                     /label=ent
     protein_bind    646..740
                     /label=5X UAS
                     /note="five tandem copies of the 'ScaI site' 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        759..997
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     protein_bind    1006..1130
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1155..1185
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             1239..1895
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             2240..2542
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(2689..2709)
                     /label=attB2
                     /note="core recombination site for the Gateway(R) BP
                     reaction"
     intron          2726..3040
                     /number=3
                     /note="RpS5"
     protein_bind    3080..3204
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(3248..3550)
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     CDS             complement(3895..4551)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(4605..4635)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     protein_bind    complement(4660..4784)
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     polyA_signal    4943..5076
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5113..5129)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5137..5153
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5161..5191)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5206..5227
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     gene            5251..9370
                     /label=mini-white
                     /note="This modified version of the white gene lacks part
                     of the first intron."
     mobile_element  complement(9370..9819)
                     /mobile_element_type="transposon:piggyBac 
                     transposableelement"
                     /label=ent
     promoter        complement(9837..9855)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9876..9892)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    9900..9916
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(9924..9954)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(9969..9990)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(10278..10866)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(11040..11897)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(11898..12002)
                     /label=AmpR promoter
     rep_origin      complement(12028..12483)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     12625..12641
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        12651..12669
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gagctcttaa ccctagaaag ataatcatat tgtgacgtac gttaaagata atcatgcgta
       61 aaattgacgc atgtgtttta tcggtctgta tatcgaggtt tatttattaa tttgaataga
      121 tattaagttt tattatattt acacttacat actaataata aattcaacaa actatttatt
      181 tatgtttatt tatttattaa aaaaaaacaa aaactcaaaa tttcttctat aaagtaacaa
      241 aacttttaaa cattctctct tttacaaaaa taaacttatt ttgtacttta aaaacagtca
      301 tgttgtatta taaaataagt aattagctta acttatacat aatagaaaca aattatactt
      361 attagtcagt cagaaacaac tttggcacat atcaatatta tgctctcgac aaataacttt
      421 tttgcatttt ttgcacgatg catttgcctt tcgccttatt ttagaggggc agtaagtaca
      481 gtaagtacgt tttttcgtta ctggctcttc agtactgtca tctgatgtac caggcacttc
      541 atttggcaaa atattagaga tattatcgcg caaatatctc ttcaaagtag gagcttctaa
      601 acgcttacgc ataaacgatg acgtcccgcg ggcatgcctg caggtcggag tactgtcctc
      661 cgagcggagt actgtcctcc gagcggagta ctgtcctccg agcggagtac tgtcctccga
      721 gcggagtact gtcctccgag cggagactct agcgagcgcc ggagtataaa tagaggcgct
      781 tcgtctacgg agcgacaatt caattcaaac aagcaaagtg aacacgtcgc taagcgaaag
      841 ctaagcaaat aaacaagcgc agctgaacaa gctaaacaat ctgcagtaaa gtgcaagtta
      901 aagtgaatca attaaaagta accagcaacc aagtaaatca actgcaacta ctgaaatctg
      961 ccaagaagta attattgaat acaagaagag aactctgcgg ccatcacaag tttgtacaaa
     1021 aaagctgaac gagaaacgta aaatgatata aatatcaata tattaaatta gattttgcat
     1081 aaaaaacaga ctacataata ctgtaaaaca caacatatcc agtcactatg gcggccgcat
     1141 taggcacccc aggctttaca ctttatgctt ccggctcgta taatgtgtgg attttgagtt
     1201 aggatccggc gagattttca ggagctaagg aagctaaaat ggagaaaaaa atcactggat
     1261 ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca tttcagtcag
     1321 ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg
     1381 taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga
     1441 atgctcatcc ggaattccgt atggcaatga aagacggtga gctggtgata tgggatagtg
     1501 ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg ctctggagtg
     1561 aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg gcgtgttacg
     1621 gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc gtctcagcca
     1681 atccctgggt gagtttcacc agttttgatt taaacgtggc caatatggac aacttcttcg
     1741 cccccgtttt caccatgggc aaatattata cgcaaggcga caaggtgctg atgccgctgg
     1801 cgattcaggt tcatcatgcc gtctgtgatg gcttccatgt cggcagaatg cttaatgaat
     1861 tacaacagta ctgcgatgag tggcagggcg gggcgtaaac gcgtggatcc ggcttactaa
     1921 aagccagata acagtatgcg tatttgcgcg ctgatttttg cggtataaga atatatactg
     1981 atatgtatac ccgaagtatg tcaaaaagag gtgtgctatg aagcagcgta ttacagtgac
     2041 agttgacagc gacagctatc agttgctcaa ggcatatatg atgtcaatat ctccggtctg
     2101 gtaagcacaa ccatgcagaa tgaagcccgt cgtctgcgtg ccgaacgctg gaaagcggaa
     2161 aatcaggaag ggatggctga ggtcgcccgg tttattgaaa tgaacggctc ttttgctgac
     2221 gagaacaggg actggtgaaa tgcagtttaa ggtttacacc tataaaagag agagccgtta
     2281 tcgtctgttt gtggatgtac agagtgatat tattgacacg cccgggcgac ggatggtgat
     2341 ccccctggcc agtgcacgtc tgctgtcaga taaagtctcc cgtgaacttt acccggtggt
     2401 gcatatcggg gatgaaagct ggcgcatgat gaccaccgat atggccagtg tgccggtctc
     2461 cgttatcggg gaagaagtgg ctgatctcag ccaccgcgaa aatgacatca aaaacgccat
     2521 taacctgatg ttctggggaa tataaatgtc aggctccctt atacacagcc agtctgcagg
     2581 tcgaccatag tgactggata tgttgtgttt tacagtatta tgtagtctgt tttttatgca
     2641 aaatctaatt taatatattg atatttatat cattttacgt ttctcgttca gctttcttgt
     2701 acaaagtggt gatggccgct ctagaactag taggtgggtt tcttctgaag atttgccttc
     2761 cagatgcatg tgctcacggt ggccggtagc aatggcttgt ggttcgttta gagactgctt
     2821 gcttataaca cttggccaga acgaaaatct atctaatatc cgtagcgtca taatgtgtag
     2881 tatagccgac atttggcgta tgaaacgaag agacattatc aattgcataa cacactttca
     2941 ttttactaac gataagaacc ccaaattctt acgctgctgt gattgcgttc ataaagttac
     3001 ttaattaatt tttttccgta tatttccttt aggcactagt ggatcccccg ggctgcagga
     3061 attcgatatc aagctatcaa ccactttgta caagaaagct gaacgagaaa cgtaaaatga
     3121 tataaatatc aatatattaa attagatttt gcataaaaaa cagactacat aatactgtaa
     3181 aacacaacat atccagtcac tatggtcgac ctgcagactg gctgtgtata agggagcctg
     3241 acatttatat tccccagaac atcaggttaa tggcgttttt gatgtcattt tcgcggtggc
     3301 tgagatcagc cacttcttcc ccgataacgg agaccggcac actggccata tcggtggtca
     3361 tcatgcgcca gctttcatcc ccgatatgca ccaccgggta aagttcacgg gagactttat
     3421 ctgacagcag acgtgcactg gccaggggga tcaccatccg tcgcccgggc gtgtcaataa
     3481 tatcactctg tacatccaca aacagacgat aacggctctc tcttttatag gtgtaaacct
     3541 taaactgcat ttcaccagtc cctgttctcg tcagcaaaag agccgttcat ttcaataaac
     3601 cgggcgacct cagccatccc ttcctgattt tccgctttcc agcgttcggc acgcagacga
     3661 cgggcttcat tctgcatggt tgtgcttacc agaccggaga tattgacatc atatatgcct
     3721 tgagcaactg atagctgtcg ctgtcaactg tcactgtaat acgctgcttc atagcacacc
     3781 tctttttgac atacttcggg tatacatatc agtatatatt cttataccgc aaaaatcagc
     3841 gcgcaaatac gcatactgtt atctggcttt tagtaagccg gatccagatc tttacgcccc
     3901 gccctgccac tcatcgcagt actgttgtaa ttcattaagc attctgccga catggaagcc
     3961 atcacagacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt
     4021 ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg ccacgtttaa
     4081 atcaaaactg gtgaaactca cccagggatt ggctgagacg aaaaacatat tctcaataaa
     4141 ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg aatatatgtg
     4201 tagaaactgc cggaaatcgt cgtggtattc actccagagc gatgaaaacg tttcagtttg
     4261 ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct caccgtcttt
     4321 cattgccata cggaattccg gatgagcatt catcaggcgg gcaagaatgt gaataaaggc
     4381 cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa tatccagctg
     4441 aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat gttctttacg
     4501 atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc
     4561 cttagctcct gaaaatctcg ccggatccta actcaaaatc cacacattat acgagccgga
     4621 agcataaagt gtaaagcctg gggtgcctaa tgcggccgcc atagtgactg gatatgttgt
     4681 gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat attgatattt
     4741 atatcatttt acgtttctcg ttcagctttt ttgtacaaac ttgttgatag cttgtccgag
     4801 ggcaaaggaa tagcacgtgc tacgagattt cgattccacc gccgccttct atgaaaggtt
     4861 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     4921 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag
     4981 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     5041 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt
     5101 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     5161 caccatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     5221 cacattaatt gcgttgcgct cactctcgag ctagtatgta tgtaagttaa taaaacccat
     5281 ttttgcggaa agtagataaa aaaaacattt ttttttttta ctgcactgga tatcattgaa
     5341 cttatctgat cagttttaaa tttacttcga tccaagggta tttgatgtac caggttcttt
     5401 cgattacctc tcactcaaaa tgacattcca ctcaaagtca gcgctgtttg cctccttctc
     5461 tgtccacaga aatatcgccg tctctttcgc cgctgcgtcc gctatctctt tcgccaccgt
     5521 ttgtagcgtt acgtagcgtc aatgtccgcc ttcagttgca ttttgtcagc ggtttcgtga
     5581 cgaagctcca agcggtttac gccatcaatt aaacacaaag tgctgtgcca aaactcctct
     5641 cgcttcttat ttttgtttgt tttttgagtg attggggtgg tgattggttt tgggtgggta
     5701 agcaggggaa agtgtgaaaa atcccggcaa tgggccaaga ggatcaggag ctattaattc
     5761 gcggaggcag caaacaccca tctgccgagc atctgaacaa tgtgagtagt acatgtgcat
     5821 acatcttaag ttcacttgat ctataggaac tgcgattgca acatcaaatt gtctgcggcg
     5881 tgagaactgc gacccacaaa aatcccaaac cgcaattgca caaacaaata gtgacacgaa
     5941 acagattatt ctggtagctg ttctcgctat ataagacaat ttttgagatc atatcatgat
     6001 caagacatct aaaggcattc attttcgact atattctttt ttacaaaaaa tataacaacc
     6061 agatatttta agctgatcct agatgcacaa aaaataaata aaagtataaa cctacttcgt
     6121 aggatacttc ggggtacttt ttgttcgggg ttagatgagc ataacgcttg tagttgatat
     6181 ttgagatccc ctatcattgc agggtgacag cggagcggct tcgcagagct gcattaacca
     6241 gggcttcggg caggccaaaa actacggcac gctccggcca cccagtccgc cggaggactc
     6301 cggttcaggg agcggccaac tagccgagaa cctcacctat gcctggcaca atatggacat
     6361 ctttggggcg gtcaatcagc cgggctccgg atggcggcag ctggtcaacc ggacacgcgg
     6421 actattctgc aacgagcgac acataccggc gcccaggaaa catttgctca agaacggtga
     6481 gtttctattc gcagtcggct gatctgtgtg aaatcttaat aaagggtcca attaccaatt
     6541 tgaaactcag tttgcggcgt ggcctatccg ggcgaacttt tggccgtgat gggcagttcc
     6601 ggtgccggaa agacgaccct gctgaatgcc cttgcctttc gatcgccgca gggcatccaa
     6661 gtatcgccat ccgggatgcg actgctcaat ggccaacctg tggacgccaa ggagatgcag
     6721 gccaggtgcg cctatgtcca gcaggatgac ctctttatcg gctccctaac ggccagggaa
     6781 cacctgattt tccaagccat ggtgcggatg ccacgacatc tgacctatcg gcagcgagtg
     6841 gcccgcgtgg atcaggtgat ccaggagctt tcgctcagca aatgtcagca cacgatcatc
     6901 ggtgtgcccg gcagggtgaa aggtctgtcc ggcggagaaa ggaagcgtct ggcattcgcc
     6961 tccgaggctc taaccgatcc gccgcttctg atctgcgatg agcccacctc cggactggac
     7021 tcctttaccg cccacagcgt cgtccaggtg ctgaagaagc tgtcgcagaa gggcaagacc
     7081 gtcatcctga ccattcatca gccgtcttcc gagctgtttg agctctttga caagatcctt
     7141 ctgatggccg agggcagggt agctttcttg ggcactccca gcgaagccgt cgacttcttt
     7201 tcctagtgag ttcgatgtgt ttattaaggg tatctagtat tacataacat ctcaactcct
     7261 atccagcgtg ggtgcccagt gtcctaccaa ctacaatccg gcggactttt acgtacaggt
     7321 gttggccgtt gtgcccggac gggagatcga gtcccgtgat cggatcgcca agatatgcga
     7381 caattttgcc attagcaaag tagcccggga tatggagcag ttgttggcca ccaaaaatct
     7441 ggagaagcca ctggagcagc cggagaatgg gtacacctac aaggccacct ggttcatgca
     7501 gttccgggcg gtcctgtggc gatcctggct gtcggtgctc aaggaaccac tcctcgtaaa
     7561 agtgcgactt attcagacaa cggtgagtgg ttccagtgga aacaaatgat ataacgctta
     7621 caattcttgg aaacaaattc gctagatttt agatagaatt gcctgattcc acacccttct
     7681 tagttttttt caatgagatg tatagtttat agttttgcag aagataaata aatttcattt
     7741 aactcgcgaa tattaatgag atgcgagtaa cattttaatt tgcagatggt tgccatcttg
     7801 attggcctca tctttttggg ccaacaactc acgcaagtgg gtgtgatgaa tatcaacgga
     7861 gccatcttcc tcttcctgac caacatgacc tttcaaaacg tctttgccac gataaatgta
     7921 agtcatgttt agaatacatt tgcatttcaa taatttacta actttctaat gaatcgattc
     7981 gatttaggtg ttcacctcag agctgccagt ttttatgagg gaggcccgaa gtcgacttta
     8041 tcgctgtgac acatactttc tgggcaaaac gattgccgaa ttgccgcttt ttctcacagt
     8101 gccactggtc ttcacggcga ttgcctatcc gatgatcgga ctgcgggccg gagtgctgca
     8161 cttcttcaac tgcctggcgc tggtcactct ggtggccaat gtgtcaacgt ccttcggata
     8221 tctaatatcc tgcgccagct cctcgacctc gatggcgctg tctgtgggtc cgccggttat
     8281 cataccattc ctgctctttg gcggcttctt cttgaactcg ggctcggtgc cagtatacct
     8341 caaatggttg tcgtacctct catggttccg ttacgccaac gagggtctgc tgattaacca
     8401 atgggcggac gtggagccgg gcgaaattag ctgcacatcg tcgaacacca cgtgccccag
     8461 ttcgggcaag gtcatcccgg agacgcttaa cttctccgcc gccgatctgc cgctggacta
     8521 cgtgggtctg gccattctca tcgtgagctt ccgggtgctc gcatatctgg ctctaagact
     8581 tcgggcccga cgcaaggagt agccgacata tatccgaaat aactgcttgt tttttttttt
     8641 taccattatt accatcgtgt ttactgttta ttgccccctc aaaaagctaa tgtaattata
     8701 tttgtgccaa taaaaacaag atatgaccta tagaatacaa gtatttcccc ttcgaacatc
     8761 cccacaagta gactttggat ttgtcttcta accaaaagac ttacacacct gcatacctta
     8821 catcaaaaac tcgtttatcg ctacataaaa caccgggata tattttttat atacatactt
     8881 ttcaaatcgc gcgccctctt cataattcac ctccaccaca ccacgtttcg tagttgctct
     8941 ttcgctgtct cccacccgct ctccgcaaca cattcacctt ttgttcgacg accttggagc
     9001 gactgtcgtt agttccgcgc gattcggttc gctcaaatgg ttccgagtgg ttcatttcgt
     9061 ctcaatagaa attagtaata aatatttgta tgtacaattt atttgctcca atatatttgt
     9121 atatatttcc ctcacagcta tatttattct aatttaatat tatgactttt taaggtaatt
     9181 ttttgtgacc tgttcggagt gattagcgtt acaatttgaa ctgaaagtga catccagtgt
     9241 ttgttccttg tgtagatgca tctcaaaaaa atggtgggca taatagtgtt gtttatatat
     9301 atcaaaaata acaactataa taataagaat acatttaatt tagaaaatgc ttggatttca
     9361 ctggaactag acgtcatctt cacttacgtg atctgatatt tcactgtcag aatcctcacc
     9421 aacaagctcg tcatcgcttt gcagaagagc agagaggata tgctcatcgt ctaaagaact
     9481 acccatttta ttatatatta gtcacgatat ctataacaag aaaatatata tataataagt
     9541 tatcacgtaa gtagaacatg aaataacaat ataattatcg tatgagttaa atcttaaaag
     9601 tcacgtaaaa gataatcatg cgtcattttg actcacgcgg tcgttatagt tcaaaatcag
     9661 tgacacttac cgcattgaca agcacgcctc acgggagctc caagcggcga ctgagatgtc
     9721 ctaaacgcac agcgacggat tcgcgctatt tagaaagaga gagcaatatt tcaagaatgc
     9781 atgcgtcaat tttacgcaga ctatctttct agggttaagg tacccagctt ttgttccctt
     9841 tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     9901 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     9961 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
    10021 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
    10081 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
    10141 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
    10201 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
    10261 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
    10321 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
    10381 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
    10441 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
    10501 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
    10561 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
    10621 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
    10681 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
    10741 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
    10801 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
    10861 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
    10921 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
    10981 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
    11041 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
    11101 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
    11161 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
    11221 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
    11281 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
    11341 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
    11401 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
    11461 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
    11521 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
    11581 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
    11641 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
    11701 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
    11761 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
    11821 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
    11881 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
    11941 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
    12001 cgcacatttc cccgaaaagt gccacctaaa ttgtaagcgt taatattttg ttaaaattcg
    12061 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
    12121 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga
    12181 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
    12241 atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag
    12301 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga
    12361 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg
    12421 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg
    12481 cgtcccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
    12541 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
    12601 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcg taatacgact
    12661 cactataggg cgaattg
//
